Expression of phosphate transporters in optimized cell culture models for dental cells biomineralization

Authors

  • Laure Merametdjian INSERM UMRS_791, University of Nantes
  • Amandine David INSERM UMRS_791, University of Nantes
  • Nina Bon INSERM UMRS_791, University of Nantes
  • Greig Couasnay INSERM UMRS_791, University of Nantes
  • J Guicheux INSERM UMRS_791, University of Nantes
  • Céline Gaucher EA 2496, Paris Descartes University
  • Sarah Beck-Cormier INSERM UMRS_791, University of Nantes
  • Laurent Beck INSERM UMRS_791, University of Nantes

Keywords:

phosphate, mineralization, ALC, M2H4

Abstract

Phosphate is a key component of dental mineral composition. The physiological role of membrane proteins of dental cells is suspected to be crucial for mineralization mechanisms. Contrary to published data related to calcium, data on regulation of phosphate flux through membrane of mineralizing cells are scarce. To address this lack of data, we studied the expression of six membranous phosphate transporters in two dental cell lines: a rat odontoblastic cell line (M2H4) and a mouse ameloblastic cell line (ALC) for which we optimized the mineralizing culture conditions.

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Published

2016-06-28

Issue

Vol. 53 No. 1 (2016)

Section

Articles

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