Considerations on biocompatibility tests «in vivo» and «in vitro» of some endodontic cements

L Fonzi, V Kaistas, A Gasparoni, M Belli, L Capezzuoli


The major goal endodontic therapy has been achieved by condensing filling materials into the root canal. It’s not uncommon to find excess material into the periapical tissue. It therefore becomes obligatory to use fillings materials that have acceptable biocompatibility.

The purpose of this investigation was to obtain a «toxicity profile» of some endodontics materials and to compare our observations to results present in literature.

The gutta-percha and five endodontic filling cements were tested «in vivo» and «in vitro».

The in vivo biocompatibility involved the placement of the test material in 10 mm. Teflon tubes with an outer diameter of 1.3 mm which were implanted subcutaneously into rats. The implants were left in situ for periods of 30 and 90 days. The «hemolysis test» is designed for «in vitro» evaluations.

The istological examination showed different intensity and extent cellular responses. In some cases severe infiltration of inflammatory cell and areas with necrotis were seen.

At conclusion, the endodontics material evaluated showed slight, moderate and severe reactions; therefore a different pattern in tissue response.


endodontic materials; toxicity; biocompatibility

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