Identification and analysis of oxygenated sterols in dental cysts

Authors

  • C Sicard-Bons
  • P Bons
  • R Escola

Keywords:

oxygenated sterols, dental cyst, capillary gas chromatography, mass spectrometry

Abstract

Our work has two main aims: to identify oxygenated sterols that accompany cholesterol in dental cyst and to develop effective methods for «profile» analyses of these sterols. Attention as focused on a family of products derived from cholesterol, characterized by the presence of one or more oxygenated functions.

More than fifty of these oxysterols are known and find most of time in differents parts of the body. In the procedure, lipids are isolated from dental cyst, the fraction is trimethylsilylated and analysed by capillary gas chromatography. Sterols are identified by comparison with reference compounds. However, two sterols of particular interest, viz cholesterol α and β epoxides, are so easily produced from cholesterol (even when rigorous precautions are taken) that indirect methods of analysis are strongly advisable. An adequate degree of quantification is possible for sterols such as 26-Hydroxycholesterol (26OHCL), which do not arise significantly as artefacts. Even to verify the fraction which seems to be 26OHCL we used thin layer chromatography coupled with mass spectrometer.

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Published

2020-04-17

Issue

Vol. 36 No. 3-4 (1993)

Section

Original Research Articles

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I hereby certify that the authors of the above manuscript have all:

1. Conceived, planned, and performed the work leading to the report, or interpreted the evidence presented, or both;

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Further, I certify that:

1. This work has not been published elsewhere and is not under revision in another journal;

2. Humane procedures have been followed in the treatment of experimental animals (if applicable);

3. Investigations in humans was done in accordance with the ethical standards of the responsible committee on human experimentation or with the Helsinki Declaration (if applicable).

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5. The copyright of the article is transferred from the authors to the Bulletin du Groupement International pour la Recherche Scientifique en Stomatologie et Odontologie upon acceptance of the manuscript.