Detection of human papillomavirus DNA sequences in leucocytes: a new approach to identify hematological markers of HPV infection in patients with oral SCC

J.F Hönig, H.J Becker, U Brinck, M Korabiowska

Abstract


Squamous cell carcinoma of oral mucosa was shown to be associated with human papillomavirus (HPV) infection. The aim of this study was to find a hematological marker for HPV infection in patients with extensive HPV positive squamous cell carcinoma of the anterior oral cavity. In forty patients, referred to our clinic, suffering from histopathologically confirmed squamous cell carcinoma of the oral cavity with a tumor index T3/N2/M0 preoperative blood probes were taken. Human leucocytes were gained by FACS-lysis centrifugation. Paraffin-embedded sections of tumor tissue were deparaffinized and proteolytically digested to expose fixed target DNA.

For detection of human papillomavirus, DNA sequences 6, 11, 16, 18, 31 and 33 a non radiocative in situ hybridization technique was used (Biohid®). For the first time we demonstrated HPV DNA sequences in leucocytes of patients with HPV positive oral SCC. Furthermore we found a correlation in type between HPV DNA sequences in tumor tissue and in leucocytes. This might lead to HPV as a hematological marker for follow up of patients with oral SCC and have consequences on therapy.


Keywords


human papillomavirus; oral carcinoma; human leucocytes; carcinogenesis; hybridization

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