EFFETS IN VITRO DV TNF-ALFA DES MASTOCYTES GINGIVAUX HUMAINS SUR LA PRODUCTION DU COLLAGENE

Authors

  • L. Fonzi
  • L. Capezzuoli
  • A. Gasparoni
  • F. Trimarch
  • M. Fonzi

Keywords:

TNF-a, human gingival fibroblast, collagen synthesis

Abstract

Toe authors carried out a study on the correlations between TNF-a and fibroblasts. The purpose was to determine the capacity of TNF-a to induce a collagen production in human gingival fibroblasts "jn culture".

The cultures treated with TNF-a were obtained from fibroblasts isolated from pathological human gingives.

Collagen production indexes used were: -(3H)Proline incorporation - Protein synthesis - DNA synthesis Deterrninations on the cultures were canied out after a short/long treatment with TNF-a against controls on cultures not treated with TNF-a. The results obtained clearly showed the positive inductive action on collagen synthesis provoked by TNF-a in gingival fibroblasts. Furthermore this action <loes not induce any increase of non-collagen proteins. The collagen synthesis only concems collagen of type l. In conclusion, it appears that TNF-a can have an important role in maintaining gingival tissue integrity.

A-2 M: a-2 macroglobulin - EBM: Eagle's basal medium - EGF: epidermal growth factor - ELAM: endothelial leukocyte adhesion molecule - ICAM: intercellular adhesion molecule - INFy : interferon y - IL: interleukin - MMPs: matrix metalloproteinases - PG E2: prostaglandin E2 - TGFB: trasforrning growth factor B - TIMPs: tissue inhibitors of metalloproteinases - TNF-a : tumor necrosis factor a - VCAM: vascular cell adhesion molecule

Downloads

Published

2021-04-06

Issue

Vol. 40 No. 4 (1998)

Section

Original Research Articles

License

I hereby certify that the authors of the above manuscript have all:

1. Conceived, planned, and performed the work leading to the report, or interpreted the evidence presented, or both;

2. Written the report or reviewed successive versions and shared in their revisions; and

3. Approved the final version.

Further, I certify that:

1. This work has not been published elsewhere and is not under revision in another journal;

2. Humane procedures have been followed in the treatment of experimental animals (if applicable);

3. Investigations in humans was done in accordance with the ethical standards of the responsible committee on human experimentation or with the Helsinki Declaration (if applicable).

4. This paper has been carefully read by a native English speaker who is familiar with the field of work (this applies to authors who are not fluent in English); and

5. The copyright of the article is transferred from the authors to the Bulletin du Groupement International pour la Recherche Scientifique en Stomatologie et Odontologie upon acceptance of the manuscript.