EFFETS IN VITRO DV TNF-ALFA DES MASTOCYTES GINGIVAUX HUMAINS SUR LA PRODUCTION DU COLLAGENE

L. Fonzi, L. Capezzuoli, A. Gasparoni, F. Trimarch, M. Fonzi

Abstract


Toe authors carried out a study on the correlations between TNF-a and fibroblasts. The purpose was to determine the capacity of TNF-a to induce a collagen production in human gingival fibroblasts "jn culture".

The cultures treated with TNF-a were obtained from fibroblasts isolated from pathological human gingives.

Collagen production indexes used were: -(3H)Proline incorporation - Protein synthesis - DNA synthesis Deterrninations on the cultures were canied out after a short/long treatment with TNF-a against controls on cultures not treated with TNF-a. The results obtained clearly showed the positive inductive action on collagen synthesis provoked by TNF-a in gingival fibroblasts. Furthermore this action <loes not induce any increase of non-collagen proteins. The collagen synthesis only concems collagen of type l. In conclusion, it appears that TNF-a can have an important role in maintaining gingival tissue integrity.

A-2 M: a-2 macroglobulin - EBM: Eagle's basal medium - EGF: epidermal growth factor - ELAM: endothelial leukocyte adhesion molecule - ICAM: intercellular adhesion molecule - INFy : interferon y - IL: interleukin - MMPs: matrix metalloproteinases - PG E2: prostaglandin E2 - TGFB: trasforrning growth factor B - TIMPs: tissue inhibitors of metalloproteinases - TNF-a : tumor necrosis factor a - VCAM: vascular cell adhesion molecule

Keywords


TNF-a; human gingival fibroblast; collagen synthesis

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