The dumbo rat mutant: a cytogenetic, morphologic and sagittal morphometric analyzis.

Stéphane Louryan, Suhair Katerji, Marcel Rooze, Nathalie Vanmuylder


h4 style="margin: 0cm 0cm 0pt"font face="Times New Roman"spanemObjective: /em/spanspan style="font-weight: normal; font-style: normal"span /spanto study the possibility of use Dumbo rat as, experimental model for understanding abnormal craniofacial development./span/font/h4font size="3"font face="Times New Roman"strongemspanDesign: /span/em/strongspanspan /spanwe investigated craniofacial morphogenesis in the Dumbo rat by morphologic and morphometric technics. We also performed a cytogenetic study of this rat. Wistar strain was considered as control. For morphologic and morphometric studies, we used Dumbo and Wistar embryos at E15 to 21. We stained these embryos emin toto/em with alcian blue and alizarin red. The skeletons of the embryos were examined and drawn under a Lucida camera, and the following sagittal measurements were taken: zygomatic length and thickness, /spanspan style="color: black"length of the mandible and its anterior and posterior thicknesses, length of the maxillary, and petrous bone height. /spanspanStatistical analyses/spanspan /spanspanwere realized using Mann Whitney test in SPSS. For /spanspancytogenetic study, chromosome spreads were prepared from lymphocyte cultures obtained from the blood of adult /spanspanrats of both strains./span/font/font h4 style="margin: 0cm 0cm 0pt"font face="Times New Roman"emspanResults:/spanspan /spanspan style="font-weight: normal"t/span/emspan style="font-weight: normal; font-style: normal"he Dumbo embryos exhibed hypoplasia of the zygomatic, maxillar and mandibular bones, and micrognathia, evoking somespan  /spanhuman dysmorphogenesis . Moreover, the position of the preliminary ear was abnormally low. /spanspan style="font-weight: normal; font-style: normal"The differences in the measurements of the craniofacial structures between the two groups of rats are significant./spanspan style="font-weight: normal; font-style: normal" /spanspan style="font-weight: normal; font-style: normal"However, the c/spanspan style="font-weight: normal; font-style: normal"ytogenetic study/spanspan style="font-weight: normal; font-style: normal" did not reveal any/spanspan style="font-weight: normal; font-style: normal" differences between the two strains./span/font/h4font size="3"font face="Times New Roman"strongemspanConclusion:/span/em/strongspan our/spanspan data indicate that the considerable morphometric differences between the craniofacial structures of Dumbo and Wistar rats might be due to genetic mutations that are undetectable by chromosome mapping./spanspan Further histologic and genetic analyses might contribute to elucidate the early determinism of the Dumbo phenotype./spanspan/span/font/fontspanfont face="Times New Roman" size="3" /font/spanspanfont face="Times New Roman" size="3" /font/span

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