Etude immunocytochimique du développement de la cavité buccale du rat

Authors

  • A Casasco
  • A Calligaro
  • C Marchetti
  • P Poggi
  • M Casasco
  • P Menghini

Keywords:

odontostomatognathic system, cell proliferation, cell différenciation, bromodeoxyudine, immunocytochemistry

Abstract

Flow cytometry and autoradiography are extensively used for the evaluation of the number of cells in S-phase in order to study the proliferative activity within tissues and organs. The recent development of a monoclonal antibody against 5-bromo-2’deoxyuridine (BrdUrd), a nonradioactive thymidine analogue that is incorporated within DNA-synthetizing cells, allows the immunochemical detection of proliferating cells. The use of this antibody allows an immunocytochemical detection of proliferating cells «in situ» on tissue sections.

We have applied this new technique for the identification of proliferating cells within the tooth and other organs of rat mouth during development. We believe that this technique, for its precision and simplicity, may be a powerful tool for studying the citokinetic and the differentiation of normal and neoplastic cells within the mouth as well as in other districts of the body.

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Published

2020-04-17

Issue

Vol. 32 No. 1 (1989)

Section

Original Research Articles

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1. Conceived, planned, and performed the work leading to the report, or interpreted the evidence presented, or both;

2. Written the report or reviewed successive versions and shared in their revisions; and

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Further, I certify that:

1. This work has not been published elsewhere and is not under revision in another journal;

2. Humane procedures have been followed in the treatment of experimental animals (if applicable);

3. Investigations in humans was done in accordance with the ethical standards of the responsible committee on human experimentation or with the Helsinki Declaration (if applicable).

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5. The copyright of the article is transferred from the authors to the Bulletin du Groupement International pour la Recherche Scientifique en Stomatologie et Odontologie upon acceptance of the manuscript.