Biochemical study of the dental pulp of the calf

B Vernole, E Caprioglio, E Mincione, V Vannini, A Tomasi, A Bini, V Franceschini

Abstract


A biochemical study of dental pulp of calves has been performed concerning:

a) peroxydability

b) A, E, C vitamins content

c) glutation (GSH) content

d) presence of paramagnetic compounds

e) phosphorylation ratio

The dental pulp from incisives of 5 months old calves has been preserved. Immediately after decapitation the pulp was immersed in liquid nitrogen. Chromatographie (HPLC) and spectroscopic (NMR-ESR) techniques have been used.

GSH in dental pulp are present and dosable (4.56 +/-0.08 n moles/mg prot.) and GSSG (1.05 +/-0.01 n moles/mg prot.).

Because of blood traces in the extracted pulps, the AA. have determined the hemoglobin (Hb) dosage and GSH of erythrocytary derivation (Fig. 1).

After deduction of GSH of erythrocytary derivation, the GSH really present in the pulp was 4.41 n moles/mg prot. and the GSSG was 0.90 n moles/mg prot.

Peroxydability of the dental pulp has been evaluated with Lowry method with dental pulp homogenate and rat liver homogenate (see Table 1).

The ESR spectre shows 4 resonances with the following values: g. 2.24 - 2.04 - 2.00 - 1.97; there are some free intermediary radicals (gr. - 2.00) (Fig.2).

The NMR spectre shows the presence of ATP (0.22 n moles/g) of inorganic phosphate (16.58 n moles g) (Fig.3).

The pulp seems to have a lot of antioxydant factors. The next researches will be to study E, A and C vitamins concentrations. This high presence of GSH and GSSG may be an embryonary peculiarity.


Keywords


dental pulp; biochemical research; GSH Glutation; lipidic peroxydation

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