Localization of proliferating cell nuclear antigen-immunoreactivity in human dental pulp and gingiva
Keywords:
proliferating cell nuclear antigen, dental pulp, gingiva, proliferation, humanAbstract
The proliferating cell nuclear antigen (PCNA) is regarded as an operational marker of proliferating cells. We have used PC10 monoclonal antibody to PCNA to reveal proliferation sites in human dental pulp and gingiva. Intense PCNA-immunoreactivity was observed in the basal layer of the gingiva lining epithelium and within some cells of the underlying connective tissues, including some endothelial and perivascular cells. PCNA-reactive cells were scattered throughout the pulp tissue, but were particularly numerous in the peripheral part. Since PCNA is an endogenous cell cycle-related molecule, we propose that PCNA-antibodies may represent useful tools for studying cell kinetics in human oral tissues in normal as well as pathological situations, such as tumors, wound healing and inflammation.
Downloads
Published
Issue
Section
License
I hereby certify that the authors of the above manuscript have all:
1. Conceived, planned, and performed the work leading to the report, or interpreted the evidence presented, or both;
2. Written the report or reviewed successive versions and shared in their revisions; and
3. Approved the final version.
Further, I certify that:
1. This work has not been published elsewhere and is not under revision in another journal;
2. Humane procedures have been followed in the treatment of experimental animals (if applicable);
3. Investigations in humans was done in accordance with the ethical standards of the responsible committee on human experimentation or with the Helsinki Declaration (if applicable).
4. This paper has been carefully read by a native English speaker who is familiar with the field of work (this applies to authors who are not fluent in English); and
5. The copyright of the article is transferred from the authors to the Bulletin du Groupement International pour la Recherche Scientifique en Stomatologie et Odontologie upon acceptance of the manuscript.